Product Description 

Although there have been many modifications to the original formulas in efforts to produce fully defined media, salt solutions still play an important role in tissue culture. A salt solution's basic function, to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions, is as valuable today as when it was first developed a century ago .

Precautions and Disclaimer 

REAGENT For R&D use only. Not for drug, household or other uses. 

Preparation Instructions

Powdered salts are hygroscopic and should be protected from moisture. The entire contents of each package should be used immediately after opening. Preparing a concentrated salt solution is not recommended as precipitates may form. Supplements can be added prior to filtration or introduced aseptically to sterile salt solution.

Storage and Stability 

Store the dry powdered salts at 2-8 °C under dry conditions and liquid medium at 2-8 °C in the dark. Deterioration of the powdered medium may be recognized by any or all of the following: [1] color change, [2] granulation/clumping, [3] insolubility. Deterioration of the liquid medium may be recognized by any or all of the following: [1] pH change, [2] precipitate or particulates, [3] cloudy appearance [4] color change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date. Procedure Materials Required but Not Provided Water for tissue culture [W3500] Sodium Bicarbonate [S5761] or Sodium Bicarbonate Solution, 7.5% [S8761] 1N Hydrochloric Acid [H9892] 1N Sodium Hydroxide [S2770] Medium additives as required 

Reference 

1. Krebs, H. A. and Henseleit, K. (1932). Untersuchungen über die Harnstoffbildung im Tierkörper in Hoppe-Seyler's Zeitschrift für Physiol. Chemie, 210, 33-66.