50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. EpanEra's IPKine® products solve these problems through less background noise, and enhanced accuracy. Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots. EpanEra secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. 
IPKine是一系列新型的二抗分子，能有效解决IP-WB后的重链干扰问题。在免疫沉淀（Immunoprecipitation， IP）和免疫共沉淀实验中，IP和WB的操作过程中通常会用到相同的一抗或同种属来源的一抗，这种情况下，WB实验中IP抗体变性后产生的重链和轻链都能够被接下来的二抗识别， 而此非特异的信号往往会部分甚至完全掩盖目的蛋白的信号。IPKine系列二抗经过特别优化，不会与IgG重链分子的结合。
该IPKine小鼠抗兔IgG轻链二抗，HRP标记（消除重链干扰）与兔IgG分子的重链有特异性反应，经过特殊处理，与兔IgG轻链分子几乎没有交叉反应